• rss
  • facebook
  • twitter
  • linkedin

Winning Abstracts from the 2007 Medical Student Abstract Competition: Curing Diabetes and Solving the Stem Cell Dilemma: In Vitro Differentiation of Mouse Amniotic Fluid Cells into Pancreatic Islet-like Cells

James Knutson, Wake Forest University Bowman Gray School of Medicine, 2008

Blindness, end-stage renal disease, amputation, and cardiovascular disease are just a few of the well-documented effects of diabetes, a disease that affects about 20 million Americans. Embryonic stem cells may hold a cure, but research is limited by ethical concerns. A line of amniotic fluid cells expressing the stem cell marker CD117 might be a pluripotent alternative to embryonic stem cells.

Using stem cell differentiation protocols and pancreatic embryology, we hypothesize that amniotic fluid cells can differentiate into pancreatic islet-like cells. We also hypothesize that these cells can restore glycemic control in a diabetic mouse model.

Mouse amniotic fluid cells expressing CD117 were isolated and transduced with pdx-1, an important gene in pancreatic islet cell development. Western blot and RT-PCR of Pax6 (a gene controlled by PDX-1) were performed to examine PDX-1 protein expression and functionality. Control cells were cultured in Chang media; differentiated cells received serum-free culture media with basic fibroblast growth factor (bFGF). Cell morphology and insulin/glucagon production were examined. Finally, an in vivo study was performed by injecting diabetic mice with pdx-1-transduced cells and measuring blood glucose levels.

Western blot demonstrated expression of PDX-1, and RT-PCR of Pax6 showed an increase in Pax6 levels.

Control cells showed no changes in morphology and did not stain for insulin or glucagon. Differentiated cell morphology resembled pancreatic islet cells; differentiated cell aggregates stained centrally for insulin and peripherally for glucagon.

Diabetic mice receiving pdx-1-transfected cells exhibited glycemic control similar to non-diabetic mice, while diabetic mice receiving non-transduced cells lacked glycemic control.

Amniotic fluid cell differentiation into pancreatic islet-like cells was demonstrated genetically, morphologically, and functionally. In vivo studies with these cells demonstrated correction of diabetes in mice. These results suggest a possible cure for diabetes and support the possibility of using amniotic fluid cells as an embryonic stem cell alternative.

Back to August 2007 Issue of IMpact

More Articles Like This

Students: Join ACP for Free

Benefits of Membership for Students: ACP's free Medical Student Membership includes benefits designed especially to meet students' needs.

Join Now: Sign-up today and begin enjoying the benefits of ACP Medical Student Membership.

Find a Residency

Search ACP's Internal Medicine Residency Database for information on all internal medicine residency programs in the U.S. and Canada. (ACP Members only)

More search options

Share this article

Bookmark and Share

What will you learn from your Annals Virtual Patient?

Annals Virtual Patient

Annals Virtual Patients is a unique patient care simulator that mirrors real patient care decisions and consequences. CME Credit and MOC Points are available. Start off with a FREE sample case. Start your journey now.

Internal Medicine Meeting 2015 Live Simulcast!

Internal Medicine Meeting 2015 Live Simulcast

Unable to attend the meeting this year? On Saturday, May 2, seven sessions will be streamed live from the meeting. Register for the simulcast and earn CME credit after watching each session. Watch it live or download for later viewing.